Hey.
I’m trying to do a 3D reconstruction using a image stack that acquired from a light sheet scope using the 2X objective.
For some reasons It looks really nice when you check in the z projection, but they dont look nice in 3D. I’m trying to use different software to see if I can improve the quality of the 3D projection, but I’m not sure how to start or if I need any specific scrip. I have access to Imaris and Image J.