I just started using ImageJ in order to count and measure egg batches of insects but have gotten into problems when it comes to the “Analyze particles” function in ImageJ.
To give you a short summary of what I have done so far in order to count the eggs with the funciton analyze particles.
I started off with importing the picture and chaning the image type to 8bit (
image -> Type -> 8-bit) and then set the scale (
Analyze->Set scale) adjusting the threshold (
Image->Adjust->Threshold) so that the eggs are black with a white background. I then fill in holes (
Process->Binary->Fill in holes) and watershed (
Process->Binary->Watershed). The last step is the analyze particles step (
Analyze->Analyze particles) and it’s here that something is messing up. As you can see in the picture, the results tables mean,min and max values are all 255 for all the eggs. Does anyone know why this is the case and how I could fix it? If anyone have a better way of doing the egg counting, hit me up.