I am working with chromosomes and am analysing fluorescence of 3 stained areas (two above, one below). I have a macro already, that would read fluorescent peaks of 2 top points through which a short, straight line the user would draw. I now want to add parallel lines that will automatically form below it so fluorescence of the single point below it can be measured too (automatically, so users don’t have to draw them too, and so the peaks can be compared in the same macro).
I do have ideas, like using the Pythagorean Theorem; or drawing a perpendicular line, and then stacking a bunch of perpendicular lines to that perpendicular line to below it; or repeating the original line again and again; or stacking rectangles on top of each other under the original line; or simply adding parallel lines to below it. I was also thinking that I could add the command to each individual line, or add it so it works on the ROI Manager. I have no idea, however, how to do any of this.
I am using Fiji (ImageJ) and Python 3.
How would I go about doing this?
Thank you very much!!!