Hi everyone! Thank you so much for everything that you do and all of your help. For my project, I am taking images of FISH-stained brain samples and segmenting them with the Weka Segmentation tool. I have developed a Macro that counts and characterizes the particles, which works well. However, I would like to add the feature of counting all background particles outside of the cells to be able to subtract this background staining from my average particle density. I have tried using Speckle Inspector and various image manipulation techniques, but I have been unable to separate the particles inside the cells from outside. As I limit the size of cells in which particles can be counted, I can't simply subtract the total particles found from the total particles in the image. Any help is greatly appreciated!
Image of a cell with inside and outside particles: