I have this time stack of 177 frames and I want to measure the average intensity of each ROI in each frame, as shown in the image
My only concern is there are some pixels that get intersected by the ROI, and that pixel would lie in 2 different ROIs.
My question is, when I use the multi-measure tool from the ROI manager, how does this tool deal with that pixel? does it make an area weighted average of the intensity? or does it decide on which ROI should this pixel fall in, based on where the majority of its area is?
If it’s the latter, is there a way to do an area-weighted intensity measurement?