Thank you for the replies!
I am trying to extract the DAB signaling strength from a large number of tissue micro-array images (hemotoxylin-DAB stained). I want to exclude all those that don’t show any, or very low DAB signal.
I have tried the color deconvolution and another plugin, the IHC profiler, but none of them worked very well. The IHC toolbox worked perfectly and is the reason I am trying to use it.
The color deconvolution I used was the built in ImageJ, with vector set to H-DAB. Is there any preprocessing I could try to improve it? Read something about that the white balance has to be right, but not sure what to try…
Do you have any other suggestions I could try?