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Hi @Axel92Dev, and welcome to the forum!
Enhance Contrast will change the pixel values, as this macro should show:
run("Blobs (25K)"); selectWindow("blobs.gif"); run("Duplicate...", "title=blobs-enhanced.gif"); selectWindow("blobs.gif"); run("Histogram"); selectWindow("blobs-enhanced.gif"); run("Enhance Contrast...", "saturated=10 normalize"); selectWindow("blobs-enhanced.gif"); run("Histogram"); run("Tile");
If the image looks different but the histogram doesn’t change, then I’m not sure what’s going on.
To figure it out, it would be helpful to see a sample image and the exact steps you did, and the histograms before and after. It is convenient to use the macro recorder (Plugins > Macros > Record…) to keep track of your steps.
Hope this helps.
welcome to the forum!
The Enhance Contrast command does not actually alter pixel intensities but changes the so-called look-up table (LUT). That is, the mapping between intensity values and color. To propagate the changes to the pixel intensities, you have to run Image > Lookup Tables > Apply LUT.
Trying to resolve this difference in behavior, I discovered Enhance Contrast has different behaviors according to the options that are selected. In the user guide:
This command does not alter pixel values as long as Normalize, Equalize Histogram or Normalize All n Slices (in the case of stacks) are not checked.
So Enhance Contrast does the following:
- No boxes checked: only the display is enhanced. Image > Lookup Tables > Apply LUT to change pixel values.
- Normalize or Equalize checked: pixel values will be altered.
Thank you guys, you helped a lot!