I’m now using Fiji ImageJ to count the cells in 3D. I have already known how to count it in single channel images. Now I’m facing problems in dual-channel images. I split channels into green and red images after I z-stacked dozens of images and repeat the same procedure of single channel images but it didn’t show the result I want. I think it only counts the cells in the first layer of the z-stacked image. Does anyone know how to solve the problem?