I need to count the number of segments on tapeworms (or pieces of tapeworms) and I'm trying to do this is an automated way. The problem is that these segments are in the process of development so they are not exactly alike in either size or morphology (as seen from samples stained with DAPI). It's a clearly metameric pattern that the human eye is really good at picking-out. So right now, I use the cell counter feature and click on the individual segments to keep count of them. Can I use some plug-in to just upload my image and get the number of segments reported back to me?
Additionally, if I can subdivide the segments into types (immature, mature, pregravid/gravid), I would also like to be able to do that, if at all possible. There are no sharp transitions so it's a little subjective but here's my rough criteria:
immature: from the first segment to the point that there is only one clear central dot (it's the ovary)
mature: 4 dots visible and spread "linearly" (ovary and 3 testes that will continue to develop)
pregravid/gravid: hazy structure (expanded uterus, outlined in image) as testes and ovary degenerate
I've uploaded an example image of a tapeworm fragment. There's a second image where I have demarcated the rough transitions between the segment types that I would like to get. I had to reduce the file size in order to upload the images. The higher resolution files can be found at this dropbox link: https://www.dropbox.com/sh/v3s2gi6h1m4t8xj/AADdPxLS5paw6wqzV3vBCoHMa?dl=0
Anybody know how to help me?
Original tif: http://forum.imagej.net/uploads/default/original/2X/2/28d82d4e61329be9e3226d70c60c20296c1eba1c.tif
Original tif: http://forum.imagej.net/uploads/default/original/2X/f/f2febce9ee66994b1fdbc31d1704009f0b5f4b84.tif