Hi, For my lab we count rhabdomeres to quantify neurodegeneration. Rhabdomeres are the dark dots in each grouping. I circled a group of 7 rhabdomeres in blue. There are 7 in a perfect tissue sample. In a degenerated sample some of the rhabdomeres are less visible, corrupted, or not present. Can someone help create an automated or semi automated ImageJ program to quantify the number/quality of rhabdomeres per cluster, possibly by analyzing the edges. (the darkness does not relate to neurodegeneration). I also attached TIFF images depicting good quality tissue and degenerated tissue.
I really appreciate the help.