I am using the colour deconvolution plugin for my masson’s trichrome stained slides. Here are my steps:
-I use the masson’s trichrome option
-set scale to um using a reference image and checked global
-circle the area of the image I want to threshold
-measure that area
-threshold using one of the algorithms provided
-measure that area again
Then, I get a different area.
Why does thresholding produce a different area before thresholding?