I’ll try to explain with an example image:
This a hyperstack consisting of three different channels. The red and green channels were acquired simultaneously while the cyan channel is registered a few seconds after the other two. In the above dataset, I have registered the stacks of each channel on every timepoint. I have then corrected for drift over time. The problem is that even though everything now is nicely aligned and stable, the cyan channel is offset in xy compared to the other two because of the slight difference in time of acquisition and movement of the tissue.
My question is, therefore, if anyone knows a convenient way to match the cyan channel to the red channel?