Drag and drop different image intensity


#1

I have a .dv file which I import in ImageJ by Drag and Drop. However, the image seems more saturated compared to the import function via bioformat. My question is, what normalization steps are performed when I drag and drop the file compared to normal import?

Normal import via bioformat:
53

Drag and drop import:
06


#2

@tempUser

In general - we recommend users to import their images using Bio-Formats - it’s definitely the best option for being sure your images are read in correctly…

Regarding the differences you ‘see’ between the two opening methods… it most likely is a rendering issue. You can always adjust the Brightness/Contrast to check - but better would be to query the pixel values or histogram to be sure they were both identical.

Are the two images you shared really the same exact image just opened in two different ways?

eta