I have an image that contains outlines (e.g. of nuclei) saved from CellProfiler that I wish to convert to ImageJ-ROIs
The image contains the shapes on a black background, each shape has a different grayscale value:
To segment the objects and convert the Objects to ROIs I’ve tried Findmaxima, which works very well but somehow misses some objects (see two out of several examples marked in the screenshot). Its the same image with a different LUT.
Am I using the function wrong, is it maybe a bug or would there be even a better solution to achieve what I am trying to do?