I am trying to use Multiview-Reconstruction to register, deconvolve, and fuse a 5 angles SPIM dataset.
I don’t have beads with my samples. I do have, however, an independent tiff stack with beads that I could use for PSF extraction. The issue is that I am so far unable to add that tiff file to my Multiview xml dataset file.
At some point I thought that I should start the xml file already containing the SPIM stack plus the stack with beads for PSF extraction. But I cannot convince the plugin to load them together. The SPIM stack has several angles and timepoints, the psf stack does not. I am assuming that this difference in dimensions is getting in the way of loading them together on the same xml file.
Now, for the questions:
How do I add files (tiff stacks) to an already existing xml dataset?
Is it possible to have a psf from another xml dataset loaded to an existing xml dataset?
Does anyone know how to handle this one?
Thank you in advance,