How to get size distributions of continuous metal frames?

electron-microscopy
bonej
Tags: #<Tag:0x00007fb8836a95d0> #<Tag:0x00007fb8836a9170>

#1

Following is a SEM image of nanoporous metal, now we want to get the sizes distributions (ideally Normal distribution ) of metal skeletons. We have tried Plugins->BoneJ->thickness, but what we can get is mean size with deviation. So does anybody have some good suggestions?

Right-click to save tif image


#2

I can’t see the image you posted but will try anyway:

Sounds like you want to correlate skeleton branches with thickness. First step is to label individual branches: Analyze Skeleton may help you with that.

If the skeletons are separate, you can do a connected components labelling and measure each disconnected particle individually, or make a mask for the thickness measurements.


#3

Hi - now I see your image.

You can get a full histogram of thickness values from the thickness map, just by letting Thickness run and then hitting [h] on your keyboard.


#4

Many thanks for your help. It works!