I hope you can all help me with this one. I need to mask (paint white) moving cells in videos similar to this one:
As you can see, there’s not much contrast between the cell and the background. I have done this for a while using a combination of blurring and thresholding, but some videos (like this example) are prooving very very difficult. I’m currently experimenting with the Trainable Weka Segmentation, but the first run is taking hours to train, and I’m not sure it is ideal for the purpose. I only ever have a single cell in the videos, and I would be totally fine with having to identify the cell outline in the first (or a few) frames manually, and then have the software trace the cell.
Does anyone have suggestions? Help is much appreciated!