This is my first post in this forum, so please tell me if I am missing to provide the necessary information to fully understand and answer my question or if a similar question has already been answered. So far I could find no good solution for my question:
I have three images (A, B, C) of the same tissue region. A and C consist of three different channels, B of 2 different channels. The first channel of each stack has marked the nuclei of the cells and should serve as reference for overlaying them. In the end I want a picture with with channel 2 of Stack A, channel 2 of Stack B and channel 2 and 3 of Channel C.
I tried using stackreg, but it seems not to be able to handle differnt channel numbers.
Has anyone already done something similar or can help me nevertheless?