I have ome images, which I would like to analyse. Unfortunately, the images are not evenly illuminated and furthermore some reflections are present. An example image looks like this:
I want to measure at least the area of the objects (in this case bubbles). I started with some image calculcations, which turned out not that bad.
First, I applied a Gaussian filter with 2 px on the original image. Then I divided this new image by a background image I have and created a new 32 bit float image:
This resulted in this image:
But I stillhave problems by detecting the bubbles. Furthermore, the reflections (white spots) at some contours won’t letme detect a closed object, but an object with holes (which can’t be sufficiently filled with the open/close function).
Could anyone give me some advice on how to perform the automated analysis? Furthermore, bubbles touching the borderof the fieldof view should be ignored for analysis and overlaping bubbles should be ignored, too.
I would be very happy if someone could help me