Hi guys, I’m trying to analyze a plug of roots and soil. I’d like to measure root development or root coverage on the plug.
The problem is that my plug have shiny dirt that can easily be taken for root.
Steps of what I tried :
- 8 bits
- Median filter (2.0)
- Subtract background
- Otsu automatic thresholding
- Watershed (because analyze particles was not fully working without oddly)
- Analyze Particles (Without the small particles)
I think I have some “not so bad” results for the amount of tinkering I did but is there a more elegant manner to do it ? Any idea of what I should do for some more “professional” results ?
How would you get the perimeter of the plug ?
Thank you in advance,