Imperfect Overlap with the 3D Stitching Plugin

stitching
Tags: #<Tag:0x00007fd69c3277b8>

#1

I’ve run into an issue with the 3D stitching plugin where I cannot get my images to overlap at the stitch boundaries. When I use the plugin, it appears that it tiles each of my images next to one another thereby producing duplicate regions at the stitch boundaries. When using this plugin, I am providing the program with image coordinates from a text file generated by our microscope software. Do you have any advice on how I can improve the stitching? I can provide more information if necessary.

Thanks!