I have a question regarding importing nd2 files to ImageJ.
I have made ROI’s in NIS Elements, which are saved with the image in a nd2 file. When you import the nd2 file into ImageJ with the Bio-Format tool, you can choose to show the ROI’s (second column, under “metadata-viewing”). But this doesn’t work. I have tried to make separate tiiff’s of the ROI’s in NIS, but ImageJ doesn’t accept these. Does someone have experience with this, or knows a trick to get around this?