I have a series of time-lapse images from a microscopy experiment that I would like to process. They are almost identical images, however, there was a problem with the microscope during the experiment and the brightness is not the same on some images. I have to correct this to be able to do further processing.
I tried the code outlined in a previous post using HistogramMatcher:
Unfortunately, it doesn’t work. Here’s the image that I get:
Could someone help how to solve this problem? I have many images, so manual adjustment is not an option.
Basically, I need im_1.tif and im_10.tif to have (roughly) the same brightness/contrast.
Thank you very much!