We are trying to measure the distance of grean stain from the surface of a tissue (as shown in the image)
We would want the shortest distance (perpendicular to the surface) from the furthest point of green.
Any ideas would be helpful.
here is a link to a related problem:
It won't be difficult to adapt the macro code to fit your needs.
Be aware that your problem in the first place is a problem of basic mathematics/geometry and not of image processing.
Thank you for the quick response!!!