Thank you for the detailed bug report, @George_Patterson.
I was unable to reproduce the bug under the following configurations:
- MacOS 10.12.4; Fiji Is Just ImageJ 2.0.0-rc-59/1.51k; Java 1.8.0_112 (64bit); Bio-Formats 5.4.0
- MacOS 10.12.4; Fiji Is Just ImageJ 2.0.0-rc-59/1.51k; Java 1.8.0_121 (64bit); Bio-Formats 5.4.0
The missing variable is: which file format(s) are you reading? Can you share a link to a sample bug-triggering image? If you save as TIFF locally, and open that, do you experience the issue using File ▶ Import ▶ Bio-Formats on that TIFF file?
In my tests, I used a 4D TIFF file saved with ImageJ, 1GB in size, with uint8 sample values, 85 time points and 33 focal planes.