I am having some difficulties that I am hoping that you guys can help me with. I am taking 20x images of cross sections of muscles which underwent an immunohistochemical stain. This stain allowed for the distinction of four different muscle types: Type I (DAPI blue), Type IIa (Texas red), Type IIb (FITC green), and Type IIx are black. I am looking for an automated way to count the different fibers in an image. I have only found one macro that claimed to do this, but there seems to be an error in the code. Any suggestions? I am taking the images as ND.2 but converting them to TIF files. In ImageJ the pictures open as a stack, so the channels are easily split.
Thanks a ton!