I am working on detection of ceria nanoparticles by evanescent wave microscopy. To quantify the images obtained from this, I use both Imagej and DiaTrack softwares.
I am able to find the number of particles from Diatrack. However I am not able find it using imagej. I want to use imagej because it is more accurate than DiaTrack.
I have attached 2 images obtained from evanescent wave microscopy. I have also included the 3D surface plots obtained for these images from both DiaTrack and imagej. I am able to get intensity graph from DiaTrack but not from imagej. If you could let me know how to get the intensity plot from imagej it would be of great help. Thank you very much for your time and consideration.