Hello - I’m having some trouble with ellipsoid fitting in particle analyser; (Plugins > Analyze > Particle Analyser)
Am I in the right place for help or should i be over at 3D object counter? If i am in the right place … read on:
In a volume which contains many thousands of particles I have a few tens of particles (which are ~20-30 voxels) that end up being fitted with an ellipsoid which has a major axis many hundreds or even thousands of pixels in length.
e.g. the most offensive particle is 11 voxels in area but, the ellipsoid major axis is > 27k pixels
- if i crop this particle out such that it is the only particle I am trying to fit, with 20 pixels space from the edge in all directions and run it again, it will not fit an ellipse / all radii = nan
I am looking to fit an ellipse to each particle and output azimuthal angle and centroid location for each void. So far successful for >90% of features, except for a troublesome few. They are fairly easy to spot and delete from the data, but I don’t think post-processing is the correct way to deal with these anomalies, and I plan to use this protocol for hundreds of datasets
Has anyone ran into this issue before?
thank you all