I am using SEM images to determine the particle size distribution of particles of approximately 1 um size you can see here.
As my particles are not round at all I tried to use the morphological segmentation plugin of MorpholibJ.
This methode detects the borders of my particles pretty well as you can see in the following picture.
Now my problem is that I use the scale for the ratio of pixels to unit provided by the same picture with annotations and when I use Analyze -> Particle Size it gives me an empty table or just finds 3-4 very small particles while the rest is not detected at all.
I would really appriciate any help as this is very important to me and ImageJ is of course a very complex software
Thank you very much!