I am totally new to Image J. Please guide me through the step-by-step instruction to calculate bone volume fraction. I have 1200 x-ray slice images to construct 3d volume. I also not aware to build 3D volume and then how to calculate bone volume fraction.
So I am not too familiar with bone measurements myself… but I can at least point you to a tool you could potentially use for your analysis: BoneJ. I’m assuming based on what you briefly describe - that this page on Volume Fraction will be most applicable.
If you have more questions - just post again here on the Forum - we are here to help.
I just came across this plugin. Just started using it. I have few more questions on the usage and procedure to use it.
I am using X-ray scanned slice images.
- First I import all the images by “image-sequence”
- Then I create ROI using ROI manager.
Start using BoneJ, here onwards
- Optimize the Threshold
- Measure volume fraction within ROI (I assume it is taking all images into account)
I was just wondering that what is the grey value it choose to detect the bone in images and how it it define the threshold?
Please somebody light the path here