Quanitfying Oil Red O Stains


#1

Hi,

I am trying to quantify data from my ORO stained slides that have been imaged under the microscope. I used the color threshold to only select red sections of the tissue, but when I go to measurements, there is no data for %area of the slides that are stained red. Any ideas or information would be greatly appreciated. Thanks!


#2

Hi Jessica,

You can set the desired measurements to be performed in the Analyze >> Set Measurements… menu. Does this help?