Quantification of Vessel Density in Myocardium by IHC

blood-vessel
script
plugin
Tags: #<Tag:0x00007fb87d59e718> #<Tag:0x00007fb87d59e5d8> #<Tag:0x00007fb87d59e448>

#1

Hello,

I’m rather new to ImageJ and the different tools and plug-ins out there. I have stained rat myocardium with antibodies against CD31, aSMA, and von Willibrand Factor to stain arteries and capillaries. Images are I would like to 1) quantify number of vessels and 2) Vessel Diameter per 10x image. Attached is a 20x image representative of the types of images i’d like to quantify (the signal should be brighter than these)

Does anyone know a tool/script/plugin that can be used for this application?

Thank you so much!


#2

Hi SamirZaman
I am not sure whether you had a chance to read this section for particle analysis:

But before you do that you may want to do some some processing, here is a link for the entire application, it may be worthwhile to spend time in going through different function, and you can use that applicable for your images…

hope this helps…good luck!


#3

@SamirZaman

To help you get started… Here are a few links you might find helpful:

Hope this helps get you started!

eta :slight_smile: