I am trying to quantify the aggregation of THP-1 macrophage cells to various cancer cell lines. I have used DeltaVision to take images of certain positions within the petri dish every 2 minutes for 3 hours - creating a total of 91 time points. Example shown below:
Is there any way I can use Fiji/ImageJ in order to quantify the aggregation of macrophage cells towards the cancer cells? This way I can quantify the changes in aggregation between different cancer cell lines.
Thanks in advance!