Quantifying microscopy images: top 10 tips for image acquisition


#1

This is a great, recent blog post - Quantifying microscopy images: top 10 tips for image acquisition - from Cell Profiler. The 10 topics are as follows:

  1. Lossless file formats: please, no JPG!
  2. Proper exposure time: avoid saturation and lack of dynamic range
  3. Bit depth: Be sure your image file’s bit depth suits the camera’s pixel intensity range
  4. Grayscale vs. color images: save whatever you like!
  5. Magnification/resolution/binning: make good choices
  6. Illumination/background variation: correct if you can
  7. Plate types for imaging
  8. Brightfield/histology images
  9. Selection of stains
  10. Live cell imaging

With great tips/descriptions for each !!!

I hope you find it helpful!

eta :slight_smile: