I would like to put your attention on my TraJClassifier plugin for diffusion trajectory classification. It builds upon trackmate and classifies diffusion trajectories into 4 classes: normal (or free) diffusion, confined diffusion, anomalous diffusion and directed motion.
Here is a little example:
The following figure shows several cells that are gathered within the field of view (left image). While cell borders are not visible in this focal plane, nuclear envelopes stand out clearly under darkfield illumination (white arrows). A total of 246 particle trajectories were identified as either normal diffusion (red), confined diffusion (yellow), anomalous diffusion (green) or directed motion (magenta). Boxed areas (A-D) show selected cases of directed motion:
It available since a few month but I didn’t announce it because the corresponding PLOS ONE publication was not online! Now it is:
Here the corresponding wiki website: