I am attempting to size microspheres that I have imaged on TEM. The microspheres are touching multiple other microspheres and I am having difficulty segmenting/thresholding the objects to maintain the original size of the microsphere. I either have the problem of the negative space becoming the main focus of the particle analysis, or I have thresholded the image so much that the particle size is no longer accurate.
In other programs, there is the ability to automatically draw circles around an object based on the circularity of part of that that object. Is that ability available in ImageJ?