Separating video experimental conditions


I am separating calcium imaging videos where baseline is followed by a treatment. I would like to split the time series into each condition. I have been using slice remover to separate them. However, the video files are large >15GB and requires reopening the original video each time. Is there a quicker method?




If you’re using Bio-Formats to import your videos, you can select Specify range for each series as well as Crop on import and load only the required part of your dataset: