I am basically using a Hough transform plugin to detect some droplets. As output, I get a labeled map which has 0 as background and the detected circles have their labels as intensities.
I want to convert this into binary to perform particle analysis on the same but the thresholding doesn’t work.
centroid_map = WindowManager.getImage("Centroid map") IJ.setThreshold(centroid_map, 0.5, 65536); IJ.run(centroid_map, "Convert to Mask", ""); centroid_map.show()
This method when I perform manually selects all the circles but when I convert to mask, eats away a few circles. Additionally, the particle analyzer simply says “No particles detected, threshold 255-255 may not be correct.”
Any help/ suggestions?