I need to measure the length and branch-points of neurites in treated and untreated neurons. The images are phase contrast images, although the nuclei are labelled with Hoechst. I used NeuronJ for tracing the neurites and it works perfectly. However my problem is that it is manual and since we do high throughput screens, its not useful in context of analysis of large amount of images. Does anybody know how I can automate this ? Is there a tool available which I am unaware of or if I can use a trainable tool?
Looking forward to hear back. Thank you very much in advance for your help.