I have several questions regarding the TrackMate plugin for FIJI.
I’m interested in tracking cells over time using one stack (which is a raw image in a 16-bit format) and then calculating their features using a different stack (which is in 32-bit format and contains NaNs). The 32-bit stack is a ratio between two channels, after background subtraction and thresholding.
The plugin works well on the 16-bit stack but it can’t handle the NaNs in the 32-bit stack. Also, because it’s a ratio image the cell detection is biased towards higher ratios.
In addition, the cells I’m tracking are not round, and for the measurements (such as mean intensity over time) I prefer to use my own segmentation script (unless there is an option to track objects with different shapes).
Is it possible to modify the plugin so that it will work with a stack that contains NaNs?
Is it possible to export the tracks and spots calculated from one stack using the TrackMate plugin to another stack, so that I could measure the spot properties using a different stack?
I was only able to copy the overlay or to get one ROI containing all the tracks together.
More specifically, I would like to use TrackMate within a script on one stack to track the cells, and then export the track IDs and assign them to objects I detected using my own segmentation on a different stack.
In addition, is it possible to modify the plugin so that the shape of the spots will be more flexible, for example add a circularity feature (similar to the same feature in the “analyze particles” function) that can be modified? That way, objects could be segmented according to their borders (and not as spots), and the measurements could be done directly on the detected objects using the plugin.
Thanks in advance,