Hello and I'm having trouble using TrackMate within Fiji. To begin, I'm attempting to track the linear motion of particles (different shapes and speeds) using the Linear Motion LAP Tracker. However, the software traces a jagged line rather than a smooth curve of the path. I'm measuring the average speed of the particles and it's crucial the software calculates the distance traveled accurately. Thanks in advance. P.S. the picture I just posted is representative of the problem.
Screen Shot 2017-06-15 at 2.01.04 PM
To be clear, I've attempted readjusting the brightness of the particles and the different LAP techniques.
Thanks in advance!