Hello dear friends,
I am trying to count the green pixels within the red lines (blood vessels). And later the green pixels outside te red lines (inside the alveoles). So I can compare them.
Suplementary information of what I tried.
(I am not really sure if it’s the right direction)
(for red image) Image>adjust>treshold ; default ; B&W
(green image) Image>adjust>treshold
Edit>selection> create mask
(to fill blood vessels?)
Edit create selection
Image>overlay>Add selection (ctrl+B)
Image>Overlay>To ROI manager
Image>Overlay>From ROI manager
(now the selection from the red lining is on the green dots picture, but sometimes it also excludes the green dots inside the red lines. So it is not fully correct)
Set measurement>limit to treshold
I tried measure in ROI but it doesn’t give me any real information.
Thank you for your suggestions